dextran hydrodynamic radius

Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Journal of Applied Polymer Science, 72, 871-876. Webdextran hydrodynamic radius. 1905/05) for their financial support. Dextrans in Aqueous Solution. Experimental Review on Intrinsic For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Pharm Res. Journal of Controlled Release, 119, 5-24. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). (2001). 20K. * shows a significant difference between the M.W. Expert Opin Drug Deliv. (1990). Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Drug Discov Today Technol. dextran hydrodynamic Ther. Dynamics of a Polymer Solution in a Rigid Matriz. Radiofrequency ablation of malignant liver tumors. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Gums and stabilisers for the Food Industry, 5, 447-450. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Biophysical Journal, 27, 177-181. Walkenhorst, S.; Olivier, R. (2003). Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Chem. "Dextrans in Aqueous Solution. Ann Surg Oncol. 1994, 95-0355. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Table 2 compares the properties of nanoparticles coated with one or other polymer. Die Makromolekulare Chemie, 148(1), 229-238. Structural and biocompatibility properties of dextran from Guner, A.; Kibarer, G. (2001). Carbohydrate Polymers, 74, 372-378. Dilute solution properties and degree of chain branching The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Microporation was performed 2, 5, and 10 times on each sample. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. 2016;9706:97061E6. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Herwadkar A, Banga AK. Increased epidermal laser fluence through simultaneous ultrasonic microporation. Webwhittier union high school district superintendent. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. The authors declare that they have no competing interests. Ortega, A.; Garca de la Torre, J. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. CAS The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. (2007). ; Dutt, N.V.K. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). 2003;89(2):31120. Nanoparticles in magnetic resonance imaging: from simple to dual The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. J. HHS Vulnerability Disclosure, Help Environ Toxicol Pharmacol. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). They concluded that a power law behavior seems to be approached only in the small molecular weight region. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). 2013;3(5):3588605. In addition, we evaluated the regeneration of SC after microporation. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Principles of Size Exclusion Chromatography SEC (GFC Molecular weight (M v) and hydrodynamic properties can be calculated from there. Kumar SV, Tarun P, Kumar TA. The unfolding of iRFP713 in a crowded milieu - PeerJ Epub 2012 Oct 18. Biomater Res 24, 22 (2020). Cell permeabilization using a non-thermal plasma - IOPscience The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. European Polymer Journal, 37, 619-622. Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. SWC designed and coordinated the research. statement and ACS Nano. Kasaai, Mohammad R. (2007). The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. 2009;301(8):60913. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. dextran hydrodynamic radius The Mark-Houwink. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Would you like email updates of new search results? Proc. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Macromolecules, 33, 5730-5739. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Eng. Physicochemical studies of oligodextran. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Hennink, W.E. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Journal of American Chemical Society, 76 (20), 5041-5052. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Biotechnology Progress, 19, 544-548. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. 2010;4(1):117. Structure Properties of Dextran. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. Advances in Physical Chemistry, Vol. The effect of branching is best recognized from the molecular weight dependencies of []. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Journal of Chemical Education, 80(9), 1036-1038. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Biomaterials Research The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. ; Fran, H.P. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. principles of dynamic light scattering Carbohydrate Polymers, 78, 902-907. The human skins were from the back or thigh of a 24-year-old woman. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Hydrodynamic Properties of Tragacanthin. Determination of polymer structure by Gel Permeation Chromatography. Hindered diffusion of dextran and ficoll in microporous membranes. dextran Differences were considered statistically significant at p <0.05. Macromolecules, 31, 7712-7716. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Doke SK, Dhawale SC. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. 2005;22(4):5505. ; Ghosh, T.K. Ibrahim O, Munavalli GS, Dover JS. The Mark-Houwink a constant is close to 0.5 in poor solvents. WebDextran 40 Pharmaceutical Quality. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". The solution was measured using an Anton Paar DMA35N densitometer. Ahn, G.Y., Eo, HS., Kim, D. et al. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. 8580 Macromolecules 34, Notes - NIST dextran hydrodynamic radius The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58].

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